出品公司: | Promega |
---|---|
载体名称: | pGL4.14[luc2/Hygro] |
质粒类型: | 荧光素酶载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 5841 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
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pGL4.14[luc2/Hygro]载体质粒基本信息
pGL4.14[luc2/Hygro]质粒图谱载体图谱和pGL4.14[luc2/Hygro]载体序列质粒序列多克隆位点信息
pGL4.14[luc2/Hygro]质粒载体简介
pGL4.14[luc2/Hygro]质粒序列载体序列
LOCUS pGL4.14[luc2 Hygro] 5841 bp ds-DNA circular SYN 29-JUN-2013 DEFINITION Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay. ACCESSION AY864928 VERSION . KEYWORDS pGL4.14[luc2 Hygro] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5841) AUTHORS Promega TITLE Direct Submission JOURNAL Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5841 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" misc_feature 1..70 /note="MCS" /note="multiple cloning site" CDS 100..1752 /codon_start=1 /product="firefly luciferase" /note="luciferase" /note="synthetic luc2 version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal 1796..1917 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 2111..2468 /note="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 2499..3536 /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK EVGRV" polyA_signal 3560..3608 /note="synthetic polyadenylation signal" rep_origin complement(3935..4523) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4723..5583) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 5688..5736 /note="synthetic polyadenylation signal" misc_feature 5750..5841 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt 121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac 181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc 241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg 301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag 361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac 421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc 481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa 541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc 601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac 661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc 721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc 781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc 841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc 901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg 961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc 1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg 1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc 1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc 1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc 1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct 1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac 1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac 1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc 1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg 1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca 1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc 1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag 1741 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga 1801 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 1861 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 1921 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 1981 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg 2041 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt 2101 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa 2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 2221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 2341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 2401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 2461 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc gaactcaccg 2521 ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac ctgatgcagt 2581 tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc ggctatgtac 2641 tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac cgccacttcg 2701 ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc gagagcctga 2761 catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc gaaacagagc 2821 tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc gccgacctca 2881 gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac acaacctggc 2941 gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc gtgatggacg 3001 acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg gccgaagact 3061 gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc ctgaccgaca 3121 acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac agtcagtacg 3181 aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag cagactcgct 3241 acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc tacatgctgc 3301 gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac gatgctgcct 3361 gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc ggtcgcacac 3421 aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg ctggccgaca 3481 gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga gtttaaactc 3541 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt 3601 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag 3661 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 3721 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc 3781 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 3841 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 3901 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 3961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 4141 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4261 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4321 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 4381 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4441 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 4501 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 4561 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 4621 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 4681 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag 4741 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt 4801 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc 4861 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc 4921 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg 4981 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac 5041 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg 5101 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc 5161 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact 5221 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc 5281 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat 5341 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc 5401 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac 5461 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa 5521 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct 5581 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca 5641 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt 5701 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc 5761 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg 5821 caggtgccag aacatttctc t //
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